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amphiregulin  (R&D Systems)


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    Structured Review

    R&D Systems amphiregulin
    Amphiregulin, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/amphiregulin/Mouse+Amphiregulin+Biotinylated+Antibody/pm41986877-46-14-15
    Average 93 stars, based on 21 article reviews
    amphiregulin - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Staining:

    Article Title: IL-33 promotes transcriptional and metabolic adaptations of tissue-resident Th2 cells
    Article Snippet: The following fluorochrome-conjugated antibodies were used: CD45 (clone 30-F11), CD4 (clone RM4-5), TCR-β (clone H57-597), TSLPR (clone 22H9), CD44 (clone IM7), CD62L (clone MEL-14), CD198 (CCR8; clone SA214G2), CD54(ICAM; clone YN1/1.7.4), GATA3 (clone TWAJ), FOXP3 (clone FJK-16s), IL5 (TRFK5), IL13 (eBio13A), pS6 (S235/236; CST, clone D57.2.2e). .. Additionally, biotinylated antibodies were used for the staining ST2 (IL-33R) (MDB, clone DJ8) and amphiregulin (R&D Systems, #BAF989). .. Flow cytometry was performed on BD FACSymphony and analyzed using FlowJo 10.8.1.

    Article Title: IL-33 promotes transcriptional and metabolic adaptations of tissue-resident Th2 cells.
    Article Snippet: The following fluorochromeconjugated antibodies were used: CD45 (clone 30-F11), CD4 (clone RM4-5), TCR-β (clone H57-597), TSLPR (clone 22H9), CD44 (clone IM7), CD62L (clone MEL-14), CD198 (CCR8; clone SA214G2), CD54(ICAM; clone YN1/1.7.4), GATA3 (clone TWAJ), FOXP3 (clone FJK-16s), IL-5 (TRFK5), IL-13 (eBio13A), pS6 (S235/ 236; CST, clone D57.2.2e). .. Additionally, biotinylated antibodies were used for the staining ST2 (IL-33R) (MDB, clone DJ8) and amphiregulin (R&D Systems, no. BAF989). .. Flow cytometry was performed on BD FACSymphony and analyzed using FlowJo 10.8.1.

    Article Title: IL-33 promotes transcriptional and metabolic adaptations of tissue-resident Th2 cells
    Article Snippet: The following fluorochrome-conjugated antibodies were used: CD45 (clone 30-F11), CD4 (clone RM4-5), TCR-β (clone H57-597), TSLPR (clone 22H9), CD44 (clone IM7), CD62L (clone MEL-14), CD198 (CCR8; clone SA214G2), CD54(ICAM; clone YN1/1.7.4), GATA3 (clone TWAJ), FOXP3 (clone FJK-16s), IL-5 (TRFK5), IL-13 (eBio13A), pS6 (S235/236; CST, clone D57.2.2e). .. Additionally, biotinylated antibodies were used for the staining ST2 (IL-33R) (MDB, clone DJ8) and amphiregulin (R&D Systems, no. BAF989). .. Flow cytometry was performed on BD FACSymphony and analyzed using FlowJo 10.8.1.

    Cell Culture:

    Article Title: Neuronal-ILC2 interactions regulate pancreatic glucagon and glucose homeostasis.
    Article Snippet: INTRODUCTION: The endocrine pancreas is an organ that contributes to glucose metabolic homeostasis by releasing the pancreatic hormones insulin and glucagon.. Glucagon is secreted to the blood by alpha cells located in the pancreatic islet of Langerhans.. Fasting or highenergy–consumption sports activities can result in low plasma glucose concentrations and in response, glucagon secretion is stimulated to promote endogenous glucose production in the liver.

    Recombinant:

    Article Title: Neuronal-ILC2 interactions regulate pancreatic glucagon and glucose homeostasis.
    Article Snippet: INTRODUCTION: The endocrine pancreas is an organ that contributes to glucose metabolic homeostasis by releasing the pancreatic hormones insulin and glucagon.. Glucagon is secreted to the blood by alpha cells located in the pancreatic islet of Langerhans.. Fasting or highenergy–consumption sports activities can result in low plasma glucose concentrations and in response, glucagon secretion is stimulated to promote endogenous glucose production in the liver.

    Article Title: Intraocular amphiregulin and axial elongation in non-human adolescent primates.
    Article Snippet: The purpose of the experimental interventional study was to examine the influence of intraocularly applied amphiregulin, a member of the epidermal growth factor (EGF) family, on axial length in young non-human primates.. It included three non-human primates (Macaca mulatta), aged 4–6 years.. The left eyes received three intravitreal injections of amphiregulin (400ng/50 μl) in intervals of 4 weeks, while the right eyes received three intravitreal injections of phosphate buffered solution (50 μl) at the same time points.

    In Vitro:

    Article Title: Eosinophil-derived interleukin-24 compromises epithelial integrity and aggravates airway remodeling in mouse models of allergic asthma
    Article Snippet: Results were obtained on a BD FACS Calibur Flow Cytometer and analyzed with LEGENDplex software. .. For in vitro experiments, the levels of amphiregulin in the cell-cultured supernatants were determined using a mouse amphiregulin DuoSet ELISA kit (#DY989, R&D Systems), and the levels of GM-CSF were measured using Mouse Cytokine Panel 2 mix and match subpanel (#740372, BioLegend). ..

    Article Title: Eosinophil-derived interleukin-24 compromises epithelial integrity and aggravates airway remodeling in mouse models of allergic asthma.
    Article Snippet: Results were obtained on a BD FACS Calibur Flow Cytometer and analyzed with LEGENDplex software. .. For in vitro experiments, the levels of amphiregulin in the cell-cultured supernatants were determined using a mouse amphiregulin DuoSet ELISA kit (#DY989, R&D Systems), and the levels ofGM-CSFweremeasuredusingMouseCytokine Panel 2mix andmatch subpanel (#740372, BioLegend). ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Eosinophil-derived interleukin-24 compromises epithelial integrity and aggravates airway remodeling in mouse models of allergic asthma
    Article Snippet: Results were obtained on a BD FACS Calibur Flow Cytometer and analyzed with LEGENDplex software. .. For in vitro experiments, the levels of amphiregulin in the cell-cultured supernatants were determined using a mouse amphiregulin DuoSet ELISA kit (#DY989, R&D Systems), and the levels of GM-CSF were measured using Mouse Cytokine Panel 2 mix and match subpanel (#740372, BioLegend). ..

    Article Title: Eosinophil-derived interleukin-24 compromises epithelial integrity and aggravates airway remodeling in mouse models of allergic asthma.
    Article Snippet: Results were obtained on a BD FACS Calibur Flow Cytometer and analyzed with LEGENDplex software. .. For in vitro experiments, the levels of amphiregulin in the cell-cultured supernatants were determined using a mouse amphiregulin DuoSet ELISA kit (#DY989, R&D Systems), and the levels ofGM-CSFweremeasuredusingMouseCytokine Panel 2mix andmatch subpanel (#740372, BioLegend). ..

    Injection:

    Article Title: Unique adipose tissue invariant natural killer T cell subpopulations control adipocyte turnover in mice
    Article Snippet: To expand adipose iNKT cells in vivo, mice were intraperitoneally injected with α-galactosylceramide (α-GC) (1 μg/mouse, AG-CN2-0013, AdipoGen) and sacrificed after 1 week. .. Amphiregulin (1 μg/mouse, 989-AR, R&D Systems) or Gefitinib (20 mg/kg, S1025, Selleckchem) was intraperitoneally injected once a day for three consecutive days and mice were sacrificed on the next day. ..



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    Image Search Results


    CKAP2L promotes proliferation and migration of CRC cells by promoting AREG expression. (A) RNA sequencing results for transduced CRC cells. (B) Venn diagram shows the overlapping genes between transduced cells and CSE-treated HCT116 cells. (C) Relative expression of AREG in CRC cells treated with CSE detected by RT-qPCR and normalized against β-actin. (D) Relative expression levels of AREG and CKAP2L were detected by RT-qPCR and normalized against β-actin. (E) Secreted protein levels of AREG were detected by enzyme-linked immunosorbent assay. (F) Proliferation of transduced/transfected cells was measured by Cell Counting Kit 8. (G) Migration of transduced/transfected cells was measured by Transwell assay (×100 magnification). (H) Expression levels of proteins were measured by western blotting. Data were analyzed using two-way ANOVA followed by Tukey test, or unpaired Student's t-test for two groups. * P<0.05, ** P<0.01, *** P<0.001 and **** P<0.0001. AREG, amphiregulin; CKAP2L, cytoskeleton-associated protein 2-like; CRC, colorectal cancer; CSE, cigarette smoke extract; EGFR, epidermal growth factor receptor; NC, negative control; OE, overexpression; p-, phosphorylated; RT-qPCR, reverse transcription-quantitative PCR; sh, short hairpin; si, small interfering.

    Journal: International Journal of Oncology

    Article Title: Smoking promotes colorectal cancer via the CKAP2L/AREG axis

    doi: 10.3892/ijo.2026.5872

    Figure Lengend Snippet: CKAP2L promotes proliferation and migration of CRC cells by promoting AREG expression. (A) RNA sequencing results for transduced CRC cells. (B) Venn diagram shows the overlapping genes between transduced cells and CSE-treated HCT116 cells. (C) Relative expression of AREG in CRC cells treated with CSE detected by RT-qPCR and normalized against β-actin. (D) Relative expression levels of AREG and CKAP2L were detected by RT-qPCR and normalized against β-actin. (E) Secreted protein levels of AREG were detected by enzyme-linked immunosorbent assay. (F) Proliferation of transduced/transfected cells was measured by Cell Counting Kit 8. (G) Migration of transduced/transfected cells was measured by Transwell assay (×100 magnification). (H) Expression levels of proteins were measured by western blotting. Data were analyzed using two-way ANOVA followed by Tukey test, or unpaired Student's t-test for two groups. * P<0.05, ** P<0.01, *** P<0.001 and **** P<0.0001. AREG, amphiregulin; CKAP2L, cytoskeleton-associated protein 2-like; CRC, colorectal cancer; CSE, cigarette smoke extract; EGFR, epidermal growth factor receptor; NC, negative control; OE, overexpression; p-, phosphorylated; RT-qPCR, reverse transcription-quantitative PCR; sh, short hairpin; si, small interfering.

    Article Snippet: The concentration of secreted AREG in the culture medium was then measured using the Human AREG ELISA Kit (cat. no. EK0304; Wuhan Boster Biological Technology, Ltd.) according to the manufacturer's instructions.

    Techniques: Migration, Expressing, RNA Sequencing, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Transfection, Cell Counting, Transwell Assay, Western Blot, Negative Control, Over Expression, Reverse Transcription, Real-time Polymerase Chain Reaction

    CKAP2L promotes proliferation and migration of colorectal cancer cells through the STAT3/AREG/EGFR axis. (A) Expression levels of STAT3 and p-STAT3 proteins measured by western blotting. (B) Calculation of IC 50 in HCT116 cells treated with Stattic for 24 h. (C) Levels of AREG were measured by reverse transcription-quantitative PCR and enzyme-linked immunosorbent assay. (D) Migration of cells was detected by Transwell assay (×100 magnification). (E) Proliferation of cells was measured by Cell Counting Kit 8. (F) Expression levels of proteins measured by western blotting. (G) Binding peak of STAT3 on the promoter region of AREG was detected by Cistrome Data Browser, the binding motif was predicted using the JASPAR database, and the binding of STAT3 to the AREG promoter was evaluated by chromatin immunoprecipitation assay. Data were analyzed using (A and G) Unpaired Student's t-test, and (C-F) two-way ANOVA followed by Tukey test. * P<0.05, ** P<0.01, *** P<0.001 and **** P<0.0001. AREG, amphiregulin; CKAP2L, cytoskeleton-associated protein 2-like; EGFR, epidermal growth factor receptor; NC, negative control; p-, phosphorylated; sh, short hairpin; STAT3, signal transducer and activator of transcription 3; TSS, transcription start site.

    Journal: International Journal of Oncology

    Article Title: Smoking promotes colorectal cancer via the CKAP2L/AREG axis

    doi: 10.3892/ijo.2026.5872

    Figure Lengend Snippet: CKAP2L promotes proliferation and migration of colorectal cancer cells through the STAT3/AREG/EGFR axis. (A) Expression levels of STAT3 and p-STAT3 proteins measured by western blotting. (B) Calculation of IC 50 in HCT116 cells treated with Stattic for 24 h. (C) Levels of AREG were measured by reverse transcription-quantitative PCR and enzyme-linked immunosorbent assay. (D) Migration of cells was detected by Transwell assay (×100 magnification). (E) Proliferation of cells was measured by Cell Counting Kit 8. (F) Expression levels of proteins measured by western blotting. (G) Binding peak of STAT3 on the promoter region of AREG was detected by Cistrome Data Browser, the binding motif was predicted using the JASPAR database, and the binding of STAT3 to the AREG promoter was evaluated by chromatin immunoprecipitation assay. Data were analyzed using (A and G) Unpaired Student's t-test, and (C-F) two-way ANOVA followed by Tukey test. * P<0.05, ** P<0.01, *** P<0.001 and **** P<0.0001. AREG, amphiregulin; CKAP2L, cytoskeleton-associated protein 2-like; EGFR, epidermal growth factor receptor; NC, negative control; p-, phosphorylated; sh, short hairpin; STAT3, signal transducer and activator of transcription 3; TSS, transcription start site.

    Article Snippet: The concentration of secreted AREG in the culture medium was then measured using the Human AREG ELISA Kit (cat. no. EK0304; Wuhan Boster Biological Technology, Ltd.) according to the manufacturer's instructions.

    Techniques: Migration, Expressing, Western Blot, Reverse Transcription, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Transwell Assay, Cell Counting, Binding Assay, Chromatin Immunoprecipitation, Negative Control